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| Registros recuperados: 2.860 | |
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| Peng,Tianzhong; Huang,Xuedi; Hu,Suifa; Xie,Gui; Zhou,Cheng; Xiong,Jia; Liu,Rui. |
| ABSTRACT This study aimed to investigate the expression and mechanism of N- methyl -D- aspartate receptor 1 (NMDAR1) in the pathogenesis of Alzheimer disease (AD). Eighty adult Wistar rats were randomly divided into 4 groups (n=20 each) to receive an injection of 0, 5, 7 and 10 μl of 1 μg/μl amyloid-β 42 (Aβ1-42) in the hippocampus. Twenty rats in normal control group were injected with equal volume of saline. After 10 days, the hippocampus was isolated from 5 randomly selected rats in each group. The NMDAR1 protein and mRNA expression was determined by immunohistochemical staining and qRT-PCR. The aquaporin-1 (AQP-1) mRNA expression was also measured by qRT-PCR. We found that both NMDAR1 and AQP-1 expression in Aβ1-42 groups was increased in a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NMDAR1; Alzheimer disease; Aquaporin; AQP-1. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100435 |
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| Mantovani,André. |
| Leaf succulence has important physiological and ecological implications. Currently it is quantified by Delf's index (fresh weight/leaf area) and fresh weight/ dry weight ratio. Both indeces are reconsidered and a new index is proposed. Shade and sun leaves from terrestrial, hemiepiphytic and epiphytic aroids were studied. Delf's formula, which does not consider dry weight, overestimated leaf succulence. As fresh weight / dry weight ratio (fw / dw) does not consider leaf area, plants with the same fw / dw ratio were more than five times different in area. The last case was only possible with a decrease in surface / volume ratio and a increase of mesophyll thickness, components not measured by fw / dw ratio. The new index proposed here, which takes into... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leaf succulence; Water relations; Leaf anatomy; Araceae; Ecophysiology. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000100002 |
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| Rodrigues,Cristine; Vandenberghe,Luciana Porto de Souza; Teodoro,Juliana; Oss,Juliana Fraron; Pandey,Ashok; Soccol,Carlos Ricardo. |
| Gibberellic acid (GA3) is an important hormone, which controls plant's growth and development. Solid State Fermentation (SSF) allows the use of agro-industrial residues reducing the production costs. The screening of strains (four of Gibberella fujikuoroi and one of Fusarium moniliforme) and substrates (citric pulp, soy bran, sugarcane bagasse, soy husk, cassava bagasse and coffee husk) and inoculum preparation study were conducted in order to evaluate the best conditions to produce GA3 by SSF. Fermentation assays were carried out in erlenmeyers flasks at 29°C, with initial moisture of 75-80%. Different medium for inoculum production were tested in relation to cells viability and GA3 production by SSF. F. moniliforme LPB 03 and citric pulp were chosen for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gibberellic acid; Fusarium moniliforme; Solid state fermentation; Inoculum; Agro-industrial residues. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700023 |
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| Palma,Mauri Sergio Alves; Horn,Harald; Zilli,Mario; Pigatto,Gisele; Converti,Attilio. |
| This work aimed to develop a new economic enzymatic process to treat the phenolic pollutants using pure tyrosinase in stirred vessel and adopting temperature (T), pH, rotational speed (N), initial phenol (C P,o) and enzyme (C T) concentrations as independent variables. Experimental data of the residual phenol concentration (C P) were used to calculate the oxidation efficiency (η), initial oxidation rate (-r o) and time required to reach the end of reaction (t) that were selected as the responses. Under the optimal conditions (T = 45°C, pH 6.6, N = 400 rpm, C P,o = 100 ppm and C T = 50 U/mL), η was 88.1%, -r o = 10.2 mg L-1 min-1, t = 40 min. These results suggested that tyrosinase-rich crude extracts from vegetable byproducts could be quite promising. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phenols; Tyrosinase; Wastes; Enzymatic treatment. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400016 |
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| Ozdemir,Sadin; Fincan,Sema Aguloglu; Karakaya,Adem; Enez,Baris. |
| ABSTRACT The aim of this study is the production, purification, and characterisation of thermostable raw starch hydrolyzing α-amylase produced by Bacillus mojavensis SO-10. The maximum production conditions of α-amylase were found at 36th hour, 35 °C and pH 7.0. We utilized three steps to purify the thermostable α-amylase and as a result, 34-fold and 18% yield were obtained. The molecular weight of purified α-amylase was determined as 73 kD. The Km and Vmax rates were detected as 0.010 mM and 3.38 µmol min−1, respectively. This purified α-amylase exhibited the highest activity at pH 5.0-6.0 and 70 ºC and showed stability over a wide variety of pH and temperature at 4.0-8.0, and 40-50 ºC, respectively. The thermostable purified α-amylase exhibited stability... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Α-Amylase; Raw starch; Thin-Layer Chromatography (TLC); Detergents; Apple Juice Clarification. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100412 |
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| Acevedo,Francisca; Pizzul,Leticia; Castillo,María del Pilar; Rubilar,Olga; Lienqueo,María Elena; Tortella,Gonzalo; Diez,María Cristina. |
| In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: White-rot fungi; Ligninolytic enzymes; Purification; Lignocellulosic material. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013 |
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| Carnielli-Queiroz,Lorena; Fernandes,Patricia Machado Bueno; Fernandes,Antônio Alberto Ribeiro; Ventura,José Aires. |
| Abstract Pineapple (Ananas comosus var. comosus) fusariosis is an economically important fungal disease affecting the plant and its fruit. A rapid and reliable diagnosis is the base of integrated disease management practices. Fusariosis has resulted in quarantines for pineapple products in Central America, Africa and Asia. Difficulties diagnosing and correctly identifying the fungus Fusarium guttiforme, agent of the pineapple fusariosis, have led to the search for new methodologies, and for this we developed a new reliable molecular method to detect it. For diagnostic purposes, real-time PCR of elongation factor gene 1-α (ef1) was used to rapidly, specifically and sensitively diagnose F. guttiforme. A pathogenicity test was conducted with slips of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnostic; Diseases; Quarantine; Real-time PCR. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100219 |
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| Zanella,Janice; Costa,Sergio Olavo Pinto da; Zacaria,Jucimar; Echeverrigaray,Sergio. |
| Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acanthamoeba; Monocystic culture; Micromanipulation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001 |
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| Costa,Rodrigo Netto; Abreu,Clarice Lima do Canto; Presgrave,Rosaura Farias; Alves,Eloisa Nunes; Presgrave,Octavio Augusto França; Delgado,Isabella Fernandes. |
| The aim of this work was to determine the correlation between results obtained from the Draize test and from the Total Protein Content Determination (TPC) to assess the ocular irritancy potential of 20 shampoos. For TPC, two established cell lines (SIRC and 3T3) were used. The concentration that induced 50% inhibition relative to controls (IC50) was calculated for each product. Among shampoos tested with SIRC, only one had a false positive result. However, for the 3T3, three false-negative results were found. Pearson coefficient related to the in vivo value of maximum average score (MAS) was -0.58 (p=0.007) with SIRC and -0.73 (p=0.007) with 3T3. These results showed that the TPC assay was capable to predict the ocular irritant potential of shampoos, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Draize test; Ocular irritation; TPC; Shampoos; SIRC; 3T3. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600008 |
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| Paoli,Severo de; Fonseca,Adenilson de Souza da; Paoli,Flávia de; Geller,Mauro; Presta,Giuseppe Antonio; Santos-Filho,Sebastião David; Bernardo-Filho,Mário. |
| Head and neck cancer is one of the 10 most frequent cancers worldwide, with an estimated 500000 new cases diagnosed annually. Treatment of head and neck cancers require a multidisciplinary approach due their complexity and the functional and esthetic alterations that cancer can cause. The interest of the scientific community in a specific subject can be evaluated by analyzing of the number and the quality of published papers on the topic. The information obtained from PubMed (www.ncbi.nlm.nih.gov/sites/entrez) has been used as a tool in various publications to aid the evaluation of the scientific interest in specific research areas The aim of this work is to evaluate, using PubMed, the scientific interest in studies of head and neck cancer treatments such... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scientific interest; PubMed; Cancer; Surgery; Radiotherapy; Chemotherapy. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700011 |
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| Nadal,Jessica; Pedroso,Flávia de B.; Minozzo,Bruno Rodrigo; Brito,Priscilla Salles de; Farago,Paulo Vitor; Vellosa,José Carlos Rebuglio; Miyoshi,Edmar. |
| ABSTRACT Ferulic acid (FA) is a phenolic compound with well-known antioxidant potential that can be used as a promising anti-inflammatory and anti-cancer molecule. Furthermore, it has been reported to have neuroprotective activity. One of the main problems, which limit its clinical use, is its low bioavailability when administered orally. This limitation can be circumvented by changes in their structure and/or for preparing lipid-based formulations. The aim of this study was to synthesize a derivative of FA, the hexadecyl ferulate (HF). This compound would be more susceptible to pass through blood-brain barrier (BBB) due to its lipophilic character. The HF was obtained by Steglich esterification and yielded 76.77 ± 1.35%. Its structural characterization... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ABTS•+ assay; DPPH• assay; Ferulic acid derivative; Scavenger activity; Steglich esterification. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100428 |
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| Registros recuperados: 2.860 | |
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